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Looks like we have about a 3 C difference between the benchtop thermometer we use and that measurement (orbitrap is hotter). Needing to make building mods is the worst! Fans and mini splits have not been very successful for us…. Open windows at night sometimes help.
I agree with Seth and Gabby! I like having 4 different terms for these definitions (1. umbrella term, 2. spectral/first principles analysis, 3. peak picking first then large database search, 4. peak picking based on a suspect list), but I have not heard NTS vs. NTA used in this way before. Suspect screening I have heard used for definitions 3 and 4.
By ambient temperature, do you mean the ambient temperature of the lab? This is something that we struggle with as well, especially in the summer when the AC cannot keep up with the heat produced by all the turbo pumps in our instrument lab. We start to be extra cautious about running instruments – including our orbitraps – when the lab approaches 80 F (26.7 C), and don’t run above 84 F (28.9 C). We turn the electronics off on the orbitraps (maintains vacuum) when temperatures are high for days on end. If the lab reaches 90 F (32.2 C) we completely shut down (vent the MSs). Cooler temperatures and tighter temperature limits would be better, but not achievable for us several months per year. The preventative maintenance techs have let us know our lab conditions are not ideal! We definitely see more electrical problems in hotter temperatures (e.g., communication cut off between the orbitrap and/or LC and computer), and burn through turbo pumps and fans more than we should. We have had instruments (though not orbitraps) completely break down when run in the heat too much. That being said, our 2016 Q-Exactive is still hanging in there and our 2011 Exactive still works in positive mode most of the time after a lifetime of running very dirty samples in a hot lab.
